Goat anti-PAX3 Antibody |
| Principle Name | PAX3 |
| Official Symbol | PAX3 |
| All Name and Symbols | PAX3; paired box 3; CDHS; HUP2; MGC120381; MGC120382; MGC120383; MGC120384; MGC134778; WS1; paired box homeotic gene 3; paired domain gene 3; paired domain gene HuP2 |
| Accession Number(s) | NP_852122.1; NP_000429.2; NP_039230.1; NP_852123.1; NP_852124.1; NP_852126.1; NP_852125.1; NP_001120838.1 |
| Peptide Sequence | STVHQSTIPSNPD, is from internal region |
| Human Gene ID(s) | 5077 |
| Non-Human GeneID(s) | 18505 (mouse); 114502 (rat); |
| Important Comments | This antibody is expected to recognize isoforms PAX3 (NP_852122.1), PAX3d (NP_852123.1), PAX3e (NP_852124.1), PAX3g (NP_852126.1), PAX3h (NP_852125.1), and PAX3i (NP_001120838.1). |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB |
| Tested Species | Human, Mouse, Rat |
| Peptide ELISA | antibody detection limit dilution 1 to 64000. |
| Western Blot | Approx 55kDa band observed in Human Brain (Frontal Cortex) and Mouse and Rat Brain lysates (calculated MW of 53.0kDa according to Human NP_852122.1 and 52.9kDa according to Mouse NP_032807.3). Recommended concentration |
| Reference(s) | Xia SJ, Holder DD, Pawel BR, Zhang C, Barr FG, High expression of the PAX3-FKHR oncoprotein is required to promote tumorigenesis of human myoblasts. The American journal of pathology 2009 Dec 175 (6) |
