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Goat anti-ZIC3 (aa178-189) Antibody


Item#:
dAP-2590
Size Price Quantity
50.0 ug $310.00
Goat anti-ZIC3 (aa178-189) Antibody
Principle Name ZIC3 (aa178-189)
Official Symbol ZIC3
All Name and Symbols ZIC3; Zic family member 3 (odd-paired homolog, Drosophila); HTX; HTX1; ZNF203; heterotaxy 1; zinc finger protein ZIC 3; zinc finger protein of the cerebellum 3
Accession Number(s) NP_003404.1
Peptide Sequence HVDNNQVHLGLR, is from internal region
Human Gene ID(s) 7547
Non-Human GeneID(s) 22773 (mouse); 367944 (rat);
Important Comments This antibody is expected to NOT cross-react with other ZIC proteins.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, IHC
Tested Species Human
Peptide ELISA antibody detection limit dilution 1 to 64000.
Western Blot Preliminary experiments gave an approx 70kDa band in Human, Mouse and Rat Brain lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated
Immunohistochemistry In paraffin embedded Human Prostate shows nuclear staining in select secretory cells. Recommended concentration, 5-10µg/ml.
Reference(s) Hatayama M, Tomizawa T, Sakai-Kato K, Bouvagnet P, Kose S, Imamoto N, Yokoyama S, Utsunomiya-Tate N, Mikoshiba K, Kigawa T, Aruga J, Functional and structural basis of the nuclear localization signal in the ZIC3 zinc finger domain. Human molecular genetics 2008 Nov 17 (22)

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