Goat anti-MYLK Antibody |
| Principle Name | MYLK |
| Official Symbol | MYLK |
| All Name and Symbols | MYLK; myosin, light chain kinase; DKFZp686I10125; FLJ12216; KRP; MLCK; MLCK108; MLCK210; MSTP083; myosin light chain kinase; myosin, light polypeptide kinase |
| Accession Number(s) | NP_444253.3; NP_444254.3 ; NP_444255.3; NP_444256.3 |
| Peptide Sequence | KDTKNMEAKKLSKD, is from internal region |
| Human Gene ID(s) | 4638 |
| Non-Human GeneID(s) | |
| Important Comments | This antibody is expected to recognise all reported isoforms (NP_444253.3; NP_444254.3 ; NP_444255.3 and NP_444256.3). |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 8000. |
| Western Blot | Approx 150kDa band observed in Human Colon and Lung lysates (calculated MW of 197kDa according to NP_444256.3). Recommended concentration |
| Reference(s) | Graham WV, Wang F, Clayburgh DR, Cheng JX, Yoon B, Wang Y, Lin A, Turner JR. Tumor necrosis factor-induced long myosin light chain kinase transcription is regulated by differentiation-dependent signaling events. Characterization of the human long myosin light chain kinase promoter. J Biol Chem. 2006 Sep 8;281(36) |
