Goat anti-WFDC2 Antibody |
| Principle Name | WFDC2 |
| Official Symbol | WFDC2 |
| All Name and Symbols | WFDC2; WAP four-disulfide core domain 2; EDDM4; HE4; MGC57529; WAP5; dJ461P17.6; WAP domain containing protein HE4-V4; epididymal protein 4; epididymal secretory protein E4; epididymis-specific, whey-acidic protein type, four-disulfide core; major epididy |
| Accession Number(s) | NP_006094.3 |
| Peptide Sequence | PNDKEGSCPQVNIN, is from internal region |
| Human Gene ID(s) | 10406 |
| Non-Human GeneID(s) | |
| Important Comments | |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA |
| Tested Species | |
| Peptide ELISA | antibody detection limit dilution 1 to 2000. |
| Western Blot | Not yet tested - our routinely used western blotting protocol does not allow detection of proteins smaller than the calculated size of 13kDa according to the precursor NP_006094.3. Therefore we cannot recommend an optimal concentration and |
| Reference(s) | Huhtinen K, Suvitie P, Hiissa J, Junnila J, Huvila J, Kujari H, Setälä M, Härkki P, Jalkanen J, Fraser J, Mäkinen J, Auranen A, Poutanen M, Perheentupa A, Serum HE4 concentration differentiates malignant ovarian tumours from ovarian endometriotic cysts. British journal of cancer 2009 Apr 100 (8) |
