Goat anti-CHRNE (aa185-197) Antibody

Item#:

dAP-2546

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-CHRNE (aa185-197) Antibody
Principle Name	CHRNE (aa185-197)
Official Symbol	CHRNE
All Name and Symbols	CHRNE; cholinergic receptor, nicotinic, epsilon; ACHRE; CMS1D; CMS1E; CMS2A; FCCMS; SCCMS; AchR epsilon subunit; acetylcholine receptor subunit epsilon; cholinergic receptor, nicotinic, epsilon polypeptide
Accession Number(s)	NP_000071.1
Peptide Sequence	DGKTINKIDIDTE, is from internal region
Human Gene ID(s)	1145
Non-Human GeneID(s)
Important Comments	The immunizing peptide represents a part of an extracellular domain
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA
Tested Species
Peptide ELISA	antibody detection limit dilution 1 to 4000.
Western Blot	Preliminary experiments in Human Heart and Skeletal Muscle lysates gave no specific signal but low background (at antibody concentration up to 1µg/ml). We would appreciate any feedback from people in the field - have any results been report
Reference(s)	Faber CG, Molenaar PC, Vles JS, Bonifati DM, Verschuuren JJ, van Doorn PA, Kuks JB, Wokke JH, Beeson D, De Baets M. AChR deficiency due to epsilon-subunit mutations