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Goat anti-Slc5a1 (rat) Antibody
Item#:
dAP-2542
Size Price Quantity
50.0 ug $310.00
Goat anti-Slc5a1 (rat) Antibody
Principle Name Slc5a1 (rat)
Official Symbol Slc5a1
All Name and Symbols Slc5a1; solute carrier family 5 (sodium/glucose cotransporter), member 1; MGC93553; Sglt1; Na(+)/glucose cotransporter 1; Solute carrier family 5, member alpha 1 (Na+/glucose cotransporter); high affinity sodium-glucose cotransporter; sodium/glucose cotra
Accession Number(s) NP_037165.1
Peptide Sequence KDTIEIDAEAPQKEK, is from internal region
Human Gene ID(s)
Non-Human GeneID(s) 25552 (rat);
Important Comments
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, WB, IHC
Tested Species Rat
Peptide ELISA antibody detection limit dilution 1 to 4000.
Western Blot Approx 75-80kDa band observed in isolated Brush Border Membranes (BBM) of female Rat Kidney and approx 80kDa in BBM of female Rat Small Intestine (calculated MW of 73.1kDa according to NP_037165.1). Recommended concentration
Immunohistochemistry Cryosections of paraformaldehyde fixed Rat Kidney shows strong staining of the brush-border in proximal straight tubules (S3 segments). Cryosections of paraformaldehyde fixed Rat Small Inteestines shows staining of the brush-border
Reference(s) Hummel CS, Lu C, Loo DD, Hirayama BA, Voss AA, Wright EM. Glucose transport by human renal Na+/D-glucose cotransporters SGLT1 and SGLT2. Am J Physiol Cell Physiol. 2011 Jan;300(1)

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