Goat anti-DOC2A (aa233-246) Antibody

Item#:

dAP-2535

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-DOC2A (aa233-246) Antibody
Principle Name	DOC2A (aa233-246)
Official Symbol	DOC2A
All Name and Symbols	DOC2A; double C2-like domains, alpha; Doc2; doc2-alpha; double C2-like domain-containing protein alpha
Accession Number(s)	NP_003577.2
Peptide Sequence	SCYLKELEQAEQGQ, is from internal region
Human Gene ID(s)	8448
Non-Human GeneID(s)	13446 (mouse); 65031 (rat);
Important Comments
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA
Tested Species
Peptide ELISA	antibody detection limit dilution 1 to 16000.
Western Blot	Preliminary experiments gave an approx 50kDa band in Human, Mouse and Rat Brain lysates after 0.3 µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculat
Reference(s)	Higashio H, Nishimura N, Ishizaki H, Miyoshi J, Orita S, Sakane A, Sasaki T. Doc2 alpha and Munc13-4 regulate Ca(2+) -dependent secretory lysosome exocytosis in mast cells. J Immunol. 2008 Apr 1;180(7)