Goat anti-MTHFR (aa369-383) Antibody

Item#:

dAP-2529

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-MTHFR (aa369-383) Antibody
Principle Name	MTHFR (aa369-383)
Official Symbol	MTHFR
All Name and Symbols	MTHFR; methylenetetrahydrofolate reductase (NAD(P)H); 5,10-methylenetetrahydrofolate reductase (NADPH); OTTHUMP00000002367; OTTHUMP00000002373; methylenetetrahydrofolate reductase
Accession Number(s)	NP_005948.3
Peptide Sequence	SRPKSYIYRTQEWDE, is from internal region
Human Gene ID(s)	4524
Non-Human GeneID(s)	17769 (mouse); 362657 (rat);
Important Comments
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB
Tested Species	Mouse
Peptide ELISA	antibody detection limit dilution 1 to 8000.
Western Blot	Approx 70kDa band observed in lysates of cell line NIH3T3 (calculated MW of 74.6kDa according to Human NP_005948.3 and Mouse NP_034970.2). Recommended concentration
Reference(s)	Fletcher O, Kessling AM. MTHFR association with arteriosclerotic vascular disease? Hum Genet. 1998 Jul;103(1)