Goat anti-cytochrome P450 1A1 (mouse) Antibody |
| Principle Name | cytochrome P450 1A1 (mouse) |
| Official Symbol | cyp1a1 |
| All Name and Symbols | cyp1a1; cytochrome P450, family 1, subfamily A, polypeptide 1; AHH; AHRR; CP11; Cyp1a2; P450-1; CYPIA1; aromatic compound inducible; cytochrome P450 1A1; cytochrome P450 family 1 subfamily a polypeptide 2; cytochrome P450 subfamily I, polypeptide 1; cytoc |
| Accession Number(s) | NP_001129531.1 |
| Peptide Sequence | QDRKLDENANVQLSD, is from internal region |
| Human Gene ID(s) | |
| Non-Human GeneID(s) | 13076 (mouse); 24296 (rat); |
| Important Comments | This antibody is not expected to cross-react with the similar P450 1A2 |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB |
| Tested Species | Human, Rat |
| Peptide ELISA | antibody detection limit dilution 1 to 64000. |
| Western Blot | Approx 55kDa band observed in Human and Rat Lung lysates (calculated MW of 58.2kDa according to Human NP_000490.1 and 59.4kDa according to Rat NP_036672.). Primary incubation was 1 hour. Recommended concentration |
| Reference(s) | Sulem P, Gudbjartsson DF, Geller F, Prokopenko I, Feenstra B, Aben KK, Franke B, den Heijer M, Kovacs P, Stumvoll M, Mägi R, Yanek LR, Becker LC, Boyd HA, Stacey SN, Walters GB, Jonasdottir A, Thorleifsson G, Holm H, Gudjonsson SA, Rafnar T, Björnsdottir Sequence variants at CYP1A1-CYP1A2 and AHR associate with coffee consumption. Hum Mol Genet. 2011 May 15;20(10) |