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Goat anti-Thyroid peroxidase Antibody
Item#:
dAP-2520
Size Price Quantity
50.0 ug $310.00
Goat anti-Thyroid peroxidase Antibody
Principle Name Thyroid peroxidase
Official Symbol TPO
All Name and Symbols TPO; thyroid peroxidase; MSA; TDH2A; TPX; OTTHUMP00000115535; OTTHUMP00000115536; thyroid microsomal antigen; thyroperoxidase
Accession Number(s) NP_000538.3; NP_783650.1; NP_783652.1; NP_783653.1
Peptide Sequence TRHVIQVSNEVVTDD, is from internal region
Human Gene ID(s) 7173
Non-Human GeneID(s)
Important Comments This antibody is expected to recognize reported isoforms a (NP_000538.3), b (NP_783650.1), d (NP_78652.1), and e (NP_783653.1).
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, WB, IHC
Tested Species Human
Peptide ELISA antibody detection limit dilution 1 to 16000.
Western Blot Approx 110kDa band observed in Human Thyroid Gland lysates (calculated MW of 103kDa according to NP_000538.3). Recommended concentration
Immunohistochemistry In paraffin embedded Human Thyroid Gland shows textured staining in the cytoplasm of epithelial cells. Recommended concentration, 5-10µg/ml.
Reference(s) Chen CR, Hamidi S, Braley-Mullen H, Nagayama Y, Bresee C, Aliesky HA, Rapoport B, McLachlan SM. Antibodies to thyroid peroxidase arise spontaneously with age in NOD.H-2h4 mice and appear after thyroglobulin antibodies. Endocrinology. 2010 Sep;151(9)

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