Goat anti-MYO1B Antibody |
| Principle Name | MYO1B |
| Official Symbol | MYO1B |
| All Name and Symbols | MYO1B; myosin IB; myr1; MMI-alpha; MMIa; MYH-1c; MYO1B variant protein; OTTHUMP00000165861; myosin I alpha; myosin-I alpha; myosin-Ib |
| Accession Number(s) | NP_001123630.1; NP_036355.2 |
| Peptide Sequence | RTVEAKQEKVSTT, is from internal region |
| Human Gene ID(s) | 4430 |
| Non-Human GeneID(s) | 17912 (mouse); 117057 (rat); |
| Important Comments | This antibody is expected to recognize both reported isoforms (NP_001123630.1; NP_036355.2). Reported variants represent identical protein |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB, IHC |
| Tested Species | Human, Mouse |
| Peptide ELISA | antibody detection limit dilution 1 to 16000. |
| Western Blot | Approx 120kDa band observed in lysates of cell line NIH3T3 (calculated MW of 125kDa according to Human NP_036355.2 and 128kDa according to Mouse NP_034993.2). Recommended concentration |
| Immunohistochemistry | In paraffin embedded Human Cerebral Cortex shows cytoplasm staining in the neuronal bodies. Recommended concentration, 5-10µg/ml. |
| Reference(s) | Laakso JM, Lewis JH, Shuman H, Ostap EM, Myosin I can act as a molecular force sensor. Science (New York, N.Y.) 2008 Jul 321 (5885) |
