Goat anti-SIM1 Antibody

Item#:

dAP-2514

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-SIM1 Antibody
Principle Name	SIM1
Official Symbol	SIM1
All Name and Symbols	SIM1; single-minded homolog 1 (Drosophila); bHLHe14; OTTHUMP00000016902; class E basic helix-loop-helix protein 14; single-minded homolog 1
Accession Number(s)	NP_005059.2
Peptide Sequence	SGDRYRTEQYQS, is from internal region
Human Gene ID(s)	6492
Non-Human GeneID(s)	20464 (mouse); 309888 (rat);
Important Comments
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB
Tested Species	Human
Peptide ELISA	antibody detection limit dilution 1 to 2000.
Western Blot	Approx 80kDa band observed in lysates of cell line HEK293 (calculated MW of 85.5kDa according to NP_005059.2). Recommended concentration
Reference(s)	Ghoussaini M, Stutzmann F, Couturier C, Vatin V, Durand E, Lecoeur C, Degraeve F, Heude B, Tauber M, Hercberg S, Levy-Marchal C, Tounian P, Weill J, Traurig M, Bogardus C, Baier LJ, Michaud JL, Froguel P, Meyre D, Analysis of the SIM1 contribution to polygenic obesity in the French population. Obesity (Silver Spring, Md.) 2010 Aug 18 (8)