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Goat anti-Atrial natriuretic factor Antibody
Item#:
dAP-2506
Size Price Quantity
50.0 ug $310.00
Goat anti-Atrial natriuretic factor Antibody
Principle Name Atrial natriuretic factor
Official Symbol NPPA
All Name and Symbols NPPA; natriuretic peptide A; ANF; ANP; ATFB6; CDD-ANF; PND; atrial natriuretic factor; atriopeptin; cardionatrin; natriuretic peptide precursor A; prepronatriodilatin
Accession Number(s) NP_006163.1
Peptide Sequence RIGAQSGLGCNSFR, is from C Terminus
Human Gene ID(s) 4878
Non-Human GeneID(s) 230899 (mouse); 24602 (rat);
Important Comments The immunizing peptide represents aa137-150 of the precursor NPPA.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, WB, IHC
Tested Species Human, Mouse, Rat
Peptide ELISA antibody detection limit dilution 1 to 32000.
Western Blot Approx 19kDa band observed in Mouse and Rat Heart lysates (calculated MW of 16.4kDa according to Human precursor NP_006163.1 and 16.6kDa accordiong to Mouse precursor NP_032751.1). Recommended concentration
Immunohistochemistry In paraffin embedded Human Heart shows vesicular staining inside the cardiac fibrils, accumulating towards the cell membrane. Recommended concentration, 5-10µg/ml.
Reference(s) Ibebuogu UN, Gladysheva IP, Houng AK, Reed GL. Decompensated heart failure is associated with reduced corin levels and decreased cleavage of pro-atrial natriuretic peptide. Circ Heart Fail. 2011 Mar 1;4(2)

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