Goat anti-GAD25 / GAD67 Antibody

Item#:

dAP-2489

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-GAD25 / GAD67 Antibody
Principle Name	GAD25 / GAD67
Official Symbol	GAD1
All Name and Symbols	GAD1; glutamate decarboxylase 1 (brain, 67kDa); FLJ45882; GAD; SCP; 67 kDa glutamic acid decarboxylase; GAD-67; OTTHUMP00000204990; OTTHUMP00000204993; OTTHUMP00000204994; OTTHUMP00000204995; glutamate decarboxylase 1; glutamate decarboxylase 67 kDa isofo
Accession Number(s)	NP_038473.2; NP_000808.2
Peptide Sequence	QRTNSLEEKSR, is from internal region
Human Gene ID(s)	2571
Non-Human GeneID(s)
Important Comments	This antibody is expected to recognize both reported isoforms (NP_038473.2; NP_000808.2).
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB
Tested Species	Mouse
Peptide ELISA	antibody detection limit dilution 1 to 8000.
Western Blot	Approx 70kDa band observed in Mouse Brain lysates (calculated MW of 66.6kDa according to Mouse NP_032103.2). Recommended concentration
Reference(s)	Wang G, Wang R, Ferris B, Salit J, Strulovici-Barel Y, Hackett NR, Crystal RG. Smoking-mediated up-regulation of GAD67 expression in the human airway epithelium Respir Res. 2010 Oct 29;11