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Goat anti-thyroid peroxidase Antibody
Item#:
dAP-2488
Size Price Quantity
50.0 ug $310.00
Goat anti-thyroid peroxidase Antibody
Principle Name thyroid peroxidase
Official Symbol TPO
All Name and Symbols TPO; thyroid peroxidase; MSA; TDH2A; TPX; OTTHUMP00000115535; OTTHUMP00000115536; thyroid microsomal antigen; thyroperoxidase
Accession Number(s) NP_000538.3; NP_783650.1; NP_783652.1; NP_783653.1
Peptide Sequence ELRCGKHQAVGTSPQ, is from C Terminus
Human Gene ID(s) 7173
Non-Human GeneID(s)
Important Comments This antibody is expected to recognize all reported isoforms (NP_000538.3; NP_783650.1; NP_783652.1; NP_783653.1). Reported variants represent identical protein
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, WB, IHC
Tested Species Human
Peptide ELISA antibody detection limit dilution 1 to 32000.
Western Blot Approx 110kDa band observed in Human Thyroid Gland lysates (calculated MW of 103kDa according to NP_000538.3). Recommended concentration
Immunohistochemistry In paraffin embedded Human Thyroid Gland shows textured staining in the cytoplasm of epithelial cells. Recommended concentration, 5-10µg/ml.
Reference(s) Chen CR, Hamidi S, Braley-Mullen H, Nagayama Y, Bresee C, Aliesky HA, Rapoport B, McLachlan SM. Antibodies to thyroid peroxidase arise spontaneously with age in NOD.H-2h4 mice and appear after thyroglobulin antibodies. Endocrinology. 2010 Sep;151(9)

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