Goat anti-CMA1 Antibody |
| Principle Name | CMA1 |
| Official Symbol | CMA1 |
| All Name and Symbols | CMA1; chymase 1, mast cell; CYH; MCT1; MGC119890; MGC119891; chymase 1 preproprotein transcript E; chymase 1 preproprotein transcript I; chymase, heart; chymase, mast cell; mast cell protease I |
| Accession Number(s) | NP_001827.1 |
| Peptide Sequence | QFRHPKYNTSTLHHD, is from internal region |
| Human Gene ID(s) | 1215 |
| Non-Human GeneID(s) | |
| Important Comments | |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB Trf |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 128000. |
| Western Blot | In transfected HEK293 transiently expressing full-length Human CMA1 (myc and DYKDDDDK tagged), a band of approx. 35kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-DYKDDDDK tag an |
| Reference(s) | Heikkilä HM, Lätti S, Leskinen MJ, Hakala JK, Kovanen PT, Lindstedt KA Activated mast cells induce endothelial cell apoptosis by a combined action of chymase and tumor necrosis factor-alpha Arterioscler Thromb Vasc Biol. 2008 Feb;28(2) |
