Goat anti-Caspase 3 Antibody

Item#:

dAP-2472

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-Caspase 3 Antibody
Principle Name	Caspase 3
Official Symbol	CASP3
All Name and Symbols	CASP3; caspase 3; caspase 3, apoptosis-related cysteine protease; HGNC
Accession Number(s)	NP_004337.2; NP_116786.1
Peptide Sequence	RDVSKEDHSKRS, is from internal region
Human Gene ID(s)	836
Non-Human GeneID(s)
Important Comments	This antibody is expected to recognise both reported isoforms (NP_004337.2 and NP_116786.1).
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB
Tested Species	Human
Peptide ELISA	antibody detection limit dilution 1 to 32000.
Western Blot	Approx 30kDa band observed in lysates of cell line MOLT4 (calculated MW of 31.6kDa according to NP_004337.2;). Recommended concentration
Reference(s)	Liadis N, Murakami K, Eweida M, Elford AR, Sheu L, Gaisano HY, Hakem R, Ohashi PS, Woo M. Caspase-3-dependent beta-cell apoptosis in the initiation of autoimmune diabetes mellitus. Mol Cell Biol. 2005 May;25(9)