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Goat anti-IDH3B (aa369-383) Antibody
Item#:
dAP-2451
Size Price Quantity
50.0 ug $310.00
Goat anti-IDH3B (aa369-383) Antibody
Principle Name IDH3B (aa369-383)
Official Symbol IDH3B
All Name and Symbols IDH3B; isocitrate dehydrogenase 3 (NAD+) beta; FLJ11043; H-IDHB; MGC903; RP46; NAD(+)-specific ICDH subunit beta; NAD+-specific ICDH; NAD+-specific isocitrate dehydrogenase b subunit; NAD+-specific isocitrate dehydrogenase beta; isocitrate dehydrogenase [
Accession Number(s) NP_008830.2; NP_777281.1
Peptide Sequence TTDFIKSVIGHLQTK, is from C Terminus
Human Gene ID(s) 3420
Non-Human GeneID(s) 170718 (mouse); 94173 (rat);
Important Comments This antibody is expected to recognize isoform a (NP_008830.2) and isoform c (NP_777281.1).
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, WB
Tested Species Mouse
Peptide ELISA antibody detection limit dilution 1 to 16000.
Western Blot Approx 40kDa band observed in lysates of Mouse fetal Kidney and fetal Skeletal Muscle and of cell line NIH3T3 (calculated MW of 42.2kDa according to Human NP_008830.2 and Mouse NP_570954.1). Recommended concentration
Reference(s) Bzymek KP, Colman RF. Role of alpha-Asp181, beta-Asp192, and gamma-Asp190 in the distinctive subunits of human NAD-specific isocitrate dehydrogenase. Biochemistry. 2007 May 8;46(18)

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