Goat anti-SON Antibody |
Principle Name | SON |
Official Symbol | SON |
All Name and Symbols | SON; SON DNA binding protein; BASS1; C21orf50; DBP-5; FLJ21099; FLJ33914; KIAA1019; NREBP; SON3; Bax antagonist selected in Saccharomyces 1; NRE-binding protein; OTTHUMP00000197908; OTTHUMP00000198934; negative regulatory element-binding protein; protein |
Accession Number(s) | NP_115571.1; NP_620305.1 |
Peptide Sequence | KSRCVSVQTDPTD, is from N Terminus |
Human Gene ID(s) | 6651 |
Non-Human GeneID(s) | 20658 (mouse); 304092 (rat); |
Important Comments | This antibody is expected to recognize reported isoforms B ( NP_115571.1) and F (NP_620305.1). |
Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
Applications | Pep ELISA, IHC |
Tested Species | Human |
Peptide ELISA | antibody detection limit dilution 1 to 32000. |
Western Blot | Preliminary experiments gave an approx 35kDa band in Human, Mouse and Rat Heart lysates after 0.3µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculate |
Immunohistochemistry | Paraffin embedded Human Small Intestine. Recommended concentration |
Reference(s) | Sharma A, Takata H, Shibahara K, Bubulya A, Bubulya PA, Son is essential for nuclear speckle organization and cell cycle progression. Molecular biology of the cell 2010 Feb 21 (4) |