Goat anti-ZIC2 Antibody |
Principle Name | ZIC2 |
Official Symbol | ZIC2 |
All Name and Symbols | ZIC2; Zic family member 2 (odd-paired homolog, Drosophila); HPE5; Zic family member 2 (odd-paired Drosophila homolog); Zinc finger protein of the cerebellum 2; zinc finger protein ZIC 2 |
Accession Number(s) | NP_009060.2 |
Peptide Sequence | HSGLSSNFNEWY, is from C Terminus |
Human Gene ID(s) | 7546 |
Non-Human GeneID(s) | 22772 (mouse); 361096 (rat); |
Important Comments | This antibody is expected to NOT cross-react with other ZIC proteins. |
Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
Applications | Pep ELISA, WB |
Tested Species | Human |
Peptide ELISA | antibody detection limit dilution 1 to 1000. |
Western Blot | In transfected RWPE1 transiently expressing Human ZIC2-Myc a band of approx. 65kDa is observed. This band is not observed in the non-transfected RWPE1, nor when GFP is transiently expressed. The calculated molecular size is 55.0kDa accordin |
Reference(s) | Sakuma K, Kasamatsu A, Yamatoji M, Yamano Y, Fushimi K, Iyoda M, Ogoshi K, Shinozuka K, Ogawara K, Shiiba M, Tanzawa H, Uzawa K, Expression status of Zic family member 2 as a prognostic marker for oral squamous cell carcinoma. Journal of cancer research and clinical oncology 2010 Apr 136 (4) |