Goat anti-NRAP Antibody |
| Principle Name | NRAP |
| Official Symbol | NRAP |
| All Name and Symbols | NRAP; nebulin-related anchoring protein |
| Accession Number(s) | NP_006166.3; NP_932326.2 |
| Peptide Sequence | DFAKSRSQFH, is from internal region |
| Human Gene ID(s) | 4892 |
| Non-Human GeneID(s) | 18175 (mouse); 307982 (rat); |
| Important Comments | This antibody is expected to recognize both reported isoforms (NP_006166.3; NP_932326.2). |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA |
| Tested Species | |
| Peptide ELISA | antibody detection limit dilution 1 to 32000. |
| Western Blot | Preliminary experiments gave an approx 100kDa band in Human , Mouse and Rat Heart lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculate |
| Reference(s) | Mohiddin SA, Lu S, Cardoso JP, Carroll S, Jha S, Horowits R, Fananapazir L, Genomic organization, alternative splicing, and expression of human and mouse N-RAP, a nebulin-related LIM protein of striated muscle. Cell motility and the cytoskeleton 2003 Jul 55 (3) |
