Goat anti-Hes7 Antibody |
| Principle Name | Hes7 |
| Official Symbol | Hes7 |
| All Name and Symbols | Hes7; hairy and enhancer of split 7 (Drosophila); bHLHb37; OTTMUSP00000006176; bHLH factor Hes7; mHes7; transcription factor HES-7 |
| Accession Number(s) | NP_149030.2 |
| Peptide Sequence | PRSPGQDAEALASCY, is from internal region |
| Human Gene ID(s) | 84667 |
| Non-Human GeneID(s) | 84653 (mouse); 305811 (rat); |
| Important Comments | |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA |
| Tested Species | |
| Peptide ELISA | antibody detection limit dilution 1 to 16000. |
| Western Blot | Preliminary experiments in Mouse fetal Liver and fetal Skeletal Muscle lysates gave no specific signal but low background (at antibody concentration up to 1µg/ml). We would appreciate any feedback from people in the field - have any results |
| Reference(s) | Sparrow DB, Sillence D, Wouters MA, Turnpenny PD, Dunwoodie SL, Two novel missense mutations in HAIRY-AND-ENHANCER-OF-SPLIT-7 in a family with spondylocostal dysostosis. European journal of human genetics |