Goat anti-NOX1 Antibody |
| Principle Name | NOX1 |
| Official Symbol | NOX1 |
| All Name and Symbols | NOX1; NADPH oxidase 1; GP91-2; MOX1; NOH-1; NOH1; MOX-1; NADH/NADPH mitogenic oxidase subunit P65-MOX; NADPH oxidase 1 variant NOH-1L; NADPH oxidase homolog-1; NOX-1; mitogenic oxidase (pyridine nucleotide-dependent superoxide-generating); mitogenic oxida |
| Accession Number(s) | NP_008983.2; NP_39249.1 |
| Peptide Sequence | DKATDIVTGLKQK, is from internal region |
| Human Gene ID(s) | 27035 |
| Non-Human GeneID(s) | 237038 (mouse); |
| Important Comments | This antibody is expected to recognize both reported isoforms (NP_008983.2; NP_39249.1). |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 128000. |
| Western Blot | Approx 55kDa band observed in lysates of cell line epithelial colorectal adenocarcinoma and HeLa (calculated MW of 59kDa according to NP_39249.1). Recommended concentration |
| Reference(s) | Manea A, Tanase LI, Raicu M, Simionescu M, Transcriptional regulation of NADPH oxidase isoforms, Nox1 and Nox4, by nuclear factor-kappaB in human aortic smooth muscle cells. Biochemical and biophysical research communications 2010 Jun 396 (4) |
