Goat anti-NUP84 (yeast) Antibody

Item#:

dAP-2424

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-NUP84 (yeast) Antibody
Principle Name	NUP84 (yeast)
Official Symbol	NUP84
All Name and Symbols	NUP84; Nup84p; YDL116W; Nuclear pore protein NUP84; Nucleoporin NUP84
Accession Number(s)	NP_010167.1
Peptide Sequence	ELSPTYQTERFTK, is from N Terminus
Human Gene ID(s)
Non-Human GeneID(s)
Important Comments
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB
Tested Species	S. cerevisiae
Peptide ELISA	antibody detection limit dilution 1 to 8000.
Western Blot	Approx 75kDa band observed in S. cerevisiae wildtype lysates but not in the knock-out strain (calculated MW of 83.6kDa according to NP_010167.1). Recommended concentration
Reference(s)	Brohawn SG, Schwartz TU. Molecular architecture of the Nup84-Nup145C-Sec13 edge element in the nuclear pore complex lattice. Nat Struct Mol Biol. 2009 Nov;16(11)