Goat anti-CHRNA5 Antibody

Item#:

dAP-2388

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-CHRNA5 Antibody
Principle Name	CHRNA5
Official Symbol	CHRNA5
All Name and Symbols	CHRNA5; cholinergic receptor, nicotinic, alpha 5; LNCR2; Cholinergic receptor, neuronal nicotinic, alpha polypeptide-5; neuronal acetylcholine receptor subunit alpha-5; neuronal nicotinic acetylcholine receptor, alpha5 subunit
Accession Number(s)	NP_000736.2
Peptide Sequence	HVDRYFTQKEET, is from internal region
Human Gene ID(s)	1138
Non-Human GeneID(s)
Important Comments
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB
Tested Species	Human
Peptide ELISA	antibody detection limit dilution 1 to 32000.
Western Blot	Approx 55kDa band observed in lysates of cell line KELLY (calculated MW of 53.1kDa according to NP_000736.2). Recommended concentration
Reference(s)	Kuryatov A, Berrettini W, Lindstrom J. Acetylcholine receptor (AChR) a5 subunit variant associated with risk for nicotine dependence and lung cancer reduces (a4ß2)2) 2 a5 AChR function. Mol Pharmacol. 2011 Jan;79(1)