Goat anti-IDH1 (yeast) Antibody

Item#:

dAP-2377

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-IDH1 (yeast) Antibody
Principle Name	IDH1 (yeast)
Official Symbol	IDH1
All Name and Symbols	IDH1; Idh1p; YNL037C; Subunit of mitochondrial NAD(+)-dependent isocitrate dehydrogenase, which catalyzes the oxidation of isocitrate to alpha-ketoglutarate in the TCA cycle
Accession Number(s)	NP_014361.1
Peptide Sequence	EPGSRHVGLDIKGQN, is from internal region
Human Gene ID(s)
Non-Human GeneID(s)
Important Comments
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB
Tested Species	S.cerevisiae S288c
Peptide ELISA	antibody detection limit dilution 1 to 16000.
Western Blot	Approx 38kDa band observed in wildtype lysates of S.cerevisiae (calculated MW of 39.3kDa according to NP_014361.1). Recommended concentration
Reference(s)	Helbig AO, de Groot MJ, van Gestel RA, Mohammed S, de Hulster EA, Luttik MA, Daran-Lapujade P, Pronk JT, Heck AJ, Slijper M. A three-way proteomics strategy allows differential analysis of yeast mitochondrial membrane protein complexes under anaerobic and aerobic conditions. Proteomics. 2009 Oct;9(20)