Goat anti-ERO1-like (aa105-118) Antibody

Item#:

dAP-2366

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-ERO1-like (aa105-118) Antibody
Principle Name	ERO1-like (aa105-118)
Official Symbol	ERO1L
All Name and Symbols	ERO1L; ERO1-like (S. cerevisiae); ERO1-alpha; ERO1-L; ERO1-L-alpha; ERO1-like protein alpha; endoplasmic oxidoreductin-1-like protein; oxidoreductin-1-L-alpha
Accession Number(s)	NP_055399.1
Peptide Sequence	QSDEVPDGIKSASY, is from internal region
Human Gene ID(s)	30001
Non-Human GeneID(s)
Important Comments
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB
Tested Species	Human
Peptide ELISA	antibody detection limit dilution 1 to 32000.
Western Blot	Approx 70kDa band observed in lysates of cell lines A431, HeLa, Jurkat (calculated MW of 54.4kDa according to NP_055399.1). The observed molecular weight corresponds to the glycosylated form. Recommended concentration
Reference(s)	Inaba K, Masui S, Iida H, Vavassori S, Sitia R, Suzuki M. Crystal structures of human Ero1a reveal the mechanisms of regulated and targeted oxidation of PDI. EMBO J. 2010 Oct 6;29(19)