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Goat anti-MOB2 (aa128-140) Antibody
Item#:
dAP-2358
Size Price Quantity
50.0 ug $310.00
Goat anti-MOB2 (aa128-140) Antibody
Principle Name MOB2 (aa128-140)
Official Symbol MOB2
All Name and Symbols MOB2; HCCA2; HCCA2 protein; mob2 homolog; mps one binder kinase activator-like 2
Accession Number(s) NP_001165694.1; NP_443731.2
Peptide Sequence DERGKKVKCTAPQ, is from internal region
Human Gene ID(s) 81532
Non-Human GeneID(s) 101513 (mouse); 499288 (rat);
Important Comments This antibody is expected to recognize both reported isoforms (NP_001165694.1; NP_443731.2) only.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, WB, IHC
Tested Species Human
Peptide ELISA antibody detection limit dilution 1 to 128000.
Western Blot Approx 30kDa band observed in lysates of cell line Jurkat (calculated MW of 30.2kDa according to NP_001165694.1). Recommended concentration
Immunohistochemistry In paraffin embedded Human Lung shows staining of smooth muscle cells, vasculature and apical secretory granules in the epithelium of the bronchiole. Recommended concentration, 5-10µg/ml.
Reference(s) Kohler RS, Schmitz D, Cornils H, Hemmings BA, Hergovich A, Differential NDR/LATS interactions with the human MOB family reveal a negative role for human MOB2 in the regulation of human NDR kinases. Molecular and cellular biology 2010 Sep 30 (18)

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