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Goat anti-Delta-Sarcoglycan (aa245-257) Antibody
Item#:
dAP-2353
Size Price Quantity
50.0 ug $310.00
Goat anti-Delta-Sarcoglycan (aa245-257) Antibody
Principle Name Delta-Sarcoglycan (aa245-257)
Official Symbol SGCD
All Name and Symbols SGCD; sarcoglycan, delta (35kDa dystrophin-associated glycoprotein); 35DAG; CMD1L; DAGD; MGC22567; SG-delta; SGCDP; SGD; 35 kDa dystrophin-associated glycoprotein; 35kD dystrophin-associated glycoprotein; delta-SG; delta-sarcoglycan; dystrophin associated
Accession Number(s) NP_000328.2; NP_758447.1; NP_001121681.1
Peptide Sequence RLPHGSYTPTGTR, is from internal region
Human Gene ID(s) 6444
Non-Human GeneID(s) 24052 (mouse); 497892 (rat);
Important Comments This antibody is expected to recognize isoform 1 (NP_000328.2) and isoform 3 (NP_001121681.1).
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, WB, IHC
Tested Species Human, Rat
Peptide ELISA antibody detection limit dilution 1 to 16000.
Western Blot Approx 37kDa band observed in Human and Rat Heart lysates (calculated MW of 32.2kDa according to NP_000328.2). The observed molecular weight corresponds to the glycosylated form. Recommended concentration
Immunohistochemistry In paraffin embedded Human Skeletal Muscle shows sarcolemmar staining of the fibres. Recommended concentration, 3-5µg/ml.
Reference(s) Sharma P, Tran T, Stelmack GL, McNeill K, Gosens R, Mutawe MM, Unruh H, Gerthoffer WT, Halayko AJ, Expression of the dystrophin-glycoprotein complex is a marker for human airway smooth muscle phenotype maturation. American journal of physiology. Lung cellular and molecular physiology 2008 Jan 294 (1)

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