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Goat anti-Doublecortin / DCX (aa232-242) Antibody
Item#:
dAP-2352
Size Price Quantity
50.0 ug $310.00
Goat anti-Doublecortin / DCX (aa232-242) Antibody
Principle Name Doublecortin / DCX (aa232-242)
Official Symbol DCX
All Name and Symbols DCX; doublecortin; RP5-914P14.1; DBCN; DC; FLJ51296; LISX; SCLH; XLIS; OTTHUMP00000023860; OTTHUMP00000216316; doublecortex; doublin; lis-X; lissencephalin-X; neuronal migration protein doublecortin
Accession Number(s) NP_000546.2; NP_835365.1; NP_835364.1; NP_001182482.1
Peptide Sequence KTSANMKAPQS, is from internal region
Human Gene ID(s) 1641
Non-Human GeneID(s) 13193 (mouse); 84394 (rat);
Important Comments This antibody is expected to recognize all four reported isoforms (NP_000546.2; NP_835365.1; NP_835364.1; NP_001182482.1). Reported variants represent identical protein
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, WB, IHC, IF
Tested Species Human, Mouse
Peptide ELISA antibody detection limit dilution 1 to 32000.
Western Blot Approx 45kDa band observed in Mouse fetal Brain lysates (calculated MW of 49.3kDa according to Human NP_000546.2 and 40.6kDa according to Human NP_835365.1 and Mouse NP_001103692.1). Recommended concentration
Immunohistochemistry In paraffin embedded Human Cerebellum shows axonal and incidental nuclear staining. Recommended concentration
Reference(s) Evangelisti C, Florian MC, Massimi I, Dominici C, Giannini G, Galardi S, Buè MC, Massalini S, McDowell HP, Messi E, Gulino A, Farace MG, Ciafrè SA, MiR-128 up-regulation inhibits Reelin and DCX expression and reduces neuroblastoma cell motility and invasiveness. The FASEB journal

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