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Goat anti-Sirtuin 1 / SIRT1 (aa577-590) Antibody
Item#:
dAP-2348
Size Price Quantity
50.0 ug $310.00
Goat anti-Sirtuin 1 / SIRT1 (aa577-590) Antibody
Principle Name Sirtuin 1 / SIRT1 (aa577-590)
Official Symbol SIRT1
All Name and Symbols SIRT1; sirtuin 1; SIR2L1; NAD-dependent deacetylase sirtuin-1; OTTHUMP00000198111; OTTHUMP00000198112; SIR2-like protein 1; SIR2alpha; hSIR2; hSIRT1; sir2-like 1; sirtuin type 1
Accession Number(s) NP_036370.2; NP_001135970.1
Peptide Sequence EKPQEVQTSRNVES, is from internal region
Human Gene ID(s) 23411
Non-Human GeneID(s) 93759 (mouse);
Important Comments This antibody is expected to recognize both reported isoforms (NP_036370.2; NP_001135970.1).
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, WB, IHC
Tested Species Human
Peptide ELISA antibody detection limit dilution 1 to 64000.
Western Blot Approx 120kDa band observed in lysates of cell line Jurkat (calculated MW of 81.7kDa according to NP_036370.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Cheng et al, Proc Natl Ac
Immunohistochemistry In paraffin embedded Human Skin shows perinuclear staining in select keratinocytes. Recommended concentration, 3-5µg/ml.
Reference(s) Ponugoti B, Kim DH, Xiao Z, Smith Z, Miao J, Zang M, Wu SY, Chiang CM, Veenstra TD, Kemper JK, SIRT1 deacetylates and inhibits SREBP-1C activity in regulation of hepatic lipid metabolism. The Journal of biological chemistry 2010 Oct 285 (44)

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