Goat anti-CD28 Antibody |
| Principle Name | CD28 |
| Official Symbol | CD28 |
| All Name and Symbols | CD28; CD28 molecule; MGC138290; Tp44; CD28 antigen; CD28 antigen (Tp44); T-cell-specific surface glycoprotein |
| Accession Number(s) | NP_006130.1 |
| Peptide Sequence | SQQLQVYSKTGFNCD, is from internal region |
| Human Gene ID(s) | 940 |
| Non-Human GeneID(s) | |
| Important Comments | The immunizing peptide corresponds to a part of the extracellular domain. |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB, IHC |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 64000. |
| Western Blot | Approx 48kDa band observed in Human Lymph Node and Tonsil lysates (calculated MW of 25.1kDa according to NP_006130.1). The observed molecular weight corresponds to the glycosylated form. Recommended concentration |
| Immunohistochemistry | In paraffin embedded Human Lymph Node shows membrane staining in the paracortex. Recommended concentration |
| Reference(s) | Guzman VB, Yambartsev A, Goncalves-Primo A, Silva ID, Carvalho CR, Ribalta JC, Goulart LR, Shulzhenko N, Gerbase-Delima M, Morgun A, New approach reveals CD28 and IFNG gene interaction in the susceptibility to cervical cancer. Human molecular genetics 2008 Jun 17 (12) |
