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Goat anti-NEDD8 conjugating enzyme (N Terminus) Antibody
Item#:
dAP-2327
Size Price Quantity
50.0 ug $310.00
Goat anti-NEDD8 conjugating enzyme (N Terminus) Antibody
Principle Name NEDD8 conjugating enzyme (N Terminus)
Official Symbol UBE2F
All Name and Symbols UBE2F; ubiquitin-conjugating enzyme E2F (putative); MGC18120; NCE2; NEDD8 carrier protein UBE2F; NEDD8 conjugating enzyme; NEDD8 protein ligase UBE2F; NEDD8-conjugating enzyme 2; NEDD8-conjugating enzyme UBE2F; OTTHUMP00000202749; OTTHUMP00000202755; OTTH
Accession Number(s) NP_542409.1
Peptide Sequence SKLKRDDGLKGSRT, is from N Terminus
Human Gene ID(s) 140739
Non-Human GeneID(s) 67921 (mouse); 363284 (rat);
Important Comments
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, WB
Tested Species Rat
Peptide ELISA antibody detection limit dilution 1 to 32000.
Western Blot Approx 22kDa band observed in Rat Pancreas lysates (calculated MW of 21.1kDa according to Human NP_542409.1 and 18.6kDa according to Rat NP_001008382.1). Recommended concentration
Reference(s) Huang DT, Ayrault O, Hunt HW, Taherbhoy AM, Duda DM, Scott DC, Borg LA, Neale G, Murray PJ, Roussel MF, Schulman BA. E2-RING expansion of the NEDD8 cascade confers specificity to cullin modification. Mol Cell. 2009 Feb 27;33(4)

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