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Goat anti-CYB5R3 / Dia 1 Antibody
Item#:
dAP-2306
Size Price Quantity
50.0 ug $310.00
Goat anti-CYB5R3 / Dia 1 Antibody
Principle Name CYB5R3 / Dia 1
Official Symbol CYB5R3
All Name and Symbols CYB5R3; cytochrome b5 reductase 3; B5R; DIA1; NADH-cytochrome b5 reductase 3; NADH-cytochrome b5 reductase 3 membrane-bound form; NADH-cytochrome b5 reductase 3 soluble form; OTTHUMP00000198574; OTTHUMP00000198665; diaphorase-1
Accession Number(s) NP_000389.1; NP_001123291.1; NP_001165131.1
Peptide Sequence CLPNLDHVGHPTER, is from C Terminus
Human Gene ID(s) 1727
Non-Human GeneID(s)
Important Comments This antibody is expected to recognize all three reported isoforms (NP_000389.1; NP_001123291.1; NP_001165131.1).
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, WB, IHC
Tested Species Human
Peptide ELISA antibody detection limit dilution 1 to 32000.
Western Blot Approx 37kDa band observed in Human Placenta, Testis and Umbilical Cord lysates (calculated MW of 38.2kDa according to NP_001165131.1). Recommended concentration
Immunohistochemistry In paraffin embedded Human Adrenal Gland shows heavy vesicular/granular staining in the cytoplasm of medular cells. Recommended concentration, 3-5µg/ml.
Reference(s) Fernandez-Borja M, Janssen L, Verwoerd D, Hordijk P, Neefjes J, RhoB regulates endosome transport by promoting actin assembly on endosomal membranes through Dia1. J. Cell. Sci. 2005 Jun 118 (Pt 12)

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