Goat anti-SIM2 Antibody |
| Principle Name | SIM2 |
| Official Symbol | SIM2 |
| All Name and Symbols | SIM2; single-minded homolog 2 (Drosophila); MGC119447; SIM; bHLHe15; OTTHUMP00000109044; class E basic helix-loop-helix protein 15; single-minded homolog 2; single-minded homolog 2, short isoform; transcription factor SIM2 |
| Accession Number(s) | NP_005060.1; NP_033664.2 |
| Peptide Sequence | SDLLYTPSYS, is from internal region |
| Human Gene ID(s) | 6493 |
| Non-Human GeneID(s) | 20465 (mouse); 304071 (rat); |
| Important Comments | This antibody is expected to recognize all reported isoforms (NP_005060.1; NP_033664.2). |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB |
| Tested Species | Mouse |
| Peptide ELISA | antibody detection limit dilution 1 to 1000. |
| Western Blot | Approx 80kDa band observed in fetal Mouse Kidney lysates (calculated MW of 73.2kDa according to Human NP_005060.1) and 72.5kDa according to Mouse NP_035507.2. Recommended concentration |
| Reference(s) | Fei Q, Wu ZH, Zhou X, Wang H, Wang NG, Yin RF, Wang YP, Qiu GX, [Association study of SIM2 gene polymorphisms with susceptibility to congenital scoliosis in a Chinese Han population] Zhonghua yi xue za zhi 2009 Nov 89 (41) |
