Goat anti-OCT4 / POU5F1 (mouse) Antibody

Item#:

dAP-2245

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-OCT4 / POU5F1 (mouse) Antibody
Principle Name	OCT4 / POU5F1 (mouse)
Official Symbol	POU5F1
All Name and Symbols	Pou5f1; POU domain, class 5, transcription factor 1; Oct-3; Oct-3/4; Oct-4; Oct3; Oct3/4; Oct4; Otf-3; Otf-4; Otf3; Otf3-rs7; Otf3g; Otf4
Accession Number(s)	NP_038661.2;
Peptide Sequence	DRPNAVKLEKVEP, is from internal region
Human Gene ID(s)
Non-Human GeneID(s)	18999 (mouse);
Important Comments
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB
Tested Species	Human, Mouse
Peptide ELISA	antibody detection limit dilution 1 to 16000.
Western Blot	Approx 38-40kDa band observed in lysates of cell line NIH3T3 and approx. 45kDa in lysates of cell line HepG2 (calculated MW of 38.6kDa according to Human NP_002692.2 and 38.2kDa according to Mouse NP_038661.2). The observed molecular weight
Reference(s)	Liu TM, Wu YN, Guo XM, Hui JH, Lee EH, Lim B, Effects of ectopic Nanog and Oct4 overexpression on mesenchymal stem cells. Stem cells and development 2009 Sep 18 (7)