Goat anti-BIRC6 / Apollon (aa 4724 -4735) Antibody

Item#:

dAP-2239

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-BIRC6 / Apollon (aa 4724 -4735) Antibody
Principle Name	BIRC6 / Apollon (aa 4724 -4735)
Official Symbol	BIRC6
All Name and Symbols	BIRC6; baculoviral IAP repeat-containing 6; APOLLON; BRUCE; FLJ13726; FLJ13786; KIAA1289; ubiquitin-conjugating BIR-domain enzyme apollon
Accession Number(s)	NP_057336.3
Peptide Sequence	SREYDGNIRQAT, is from internal region
Human Gene ID(s)	57448
Non-Human GeneID(s)	12211 (mouse); 313876 (rat);
Important Comments
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA
Tested Species
Peptide ELISA	antibody detection limit dilution 1 to 64000.
Western Blot	Preliminary experiments gave bands at approx 90kDa and 70kDa in lysates of cell lines A431 and HeLa after 0.1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given
Reference(s)	Lopergolo A, Pennati M, Gandellini P, Orlotti NI, Poma P, Daidone MG, Folini M, Zaffaroni N, Apollon gene silencing induces apoptosis in breast cancer cells through p53 stabilisation and caspase-3 activation. British journal of cancer 2009 Mar 100 (5)