Goat anti-IGF1 Antibody |
| Principle Name | IGF1 |
| Official Symbol | IGF1 |
| All Name and Symbols | IGF1; insulin-like growth factor 1 (somatomedin C); IGF1A; IGFI; OTTHUMP00000195084; insulin-like growth factor 1 |
| Accession Number(s) | NP_001104753.1; NP_001104754.1; NP_001104755.1; NP_000609.1 |
| Peptide Sequence | RSVRAQRHTD, is from internal region |
| Human Gene ID(s) | 3479 |
| Non-Human GeneID(s) | 16000 (mouse); 24482 (rat); |
| Important Comments | This antibody is expected to recognize all reported isoforms (NP_001104753.1; NP_001104754.1; NP_001104755.1; NP_000609.1). |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB, IHC |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 32000. |
| Western Blot | Approx 15kDa band observed in Human Uterus lysates (calculated MW of 15.2kDa according to NP_001104754.1). Recommended concentration |
| Immunohistochemistry | Paraffin embedded Human Spleen. Recommended concentration |
| Reference(s) | Furundzija V, Fritzsche J, Kaufmann J, Meyborg H, Fleck E, Kappert K, Stawowy P, IGF-1 increases macrophage motility via PKC/p38-dependent alphavbeta3-integrin inside-out signaling. Biochemical and biophysical research communications 2010 Apr 394 (3) |