Goat anti-Phenylalanine Hydroxylase Antibody

Item#:

dAP-2211

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-Phenylalanine Hydroxylase Antibody
Principle Name	Phenylalanine Hydroxylase
Official Symbol	PAH
All Name and Symbols	PAH; phenylalanine hydroxylase; PH; PKU; PKU1
Accession Number(s)	NP_000268.1
Peptide Sequence	ESRPSRLKKDE, is from internal region
Human Gene ID(s)	5053
Non-Human GeneID(s)	18478 (mouse); 24616 (rat);
Important Comments
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB, IHC
Tested Species	Human, Mouse, Rat
Peptide ELISA	antibody detection limit dilution 1 to 32000.
Western Blot	Approx 48kDa band observed in Human Liver lysate and approx. 52kDa in Mouse and Rat Liver lysates (calculated MW of 51.9kDa according to NP_000268.1). Recommended concentration
Immunohistochemistry	Paraffin embedded Human Kidney. Recommended concentration
Reference(s)	Bonyadi M, Omrani O, Moghanjoghi SM, Shiva S, Mutations of the phenylalanine hydroxylase gene in Iranian Azeri Turkish patients with phenylketonuria. Genetic testing and molecular biomarkers 2010 Apr 14 (2)