Goat anti-CPLA2-zeta Antibody

Item#:

dAP-2196

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-CPLA2-zeta Antibody
Principle Name	CPLA2-zeta
Official Symbol	PLA2G4F
All Name and Symbols	PLA2G4F; phospholipase A2, group IVF; DKFZp666G192; DKFZp781B229; PLA2G4FZ; cytosolic phospholipase A2 zeta; cPLA2-zeta
Accession Number(s)	NP_998765.3
Peptide Sequence	QTAEEKAFGDFVINR, is from internal region (near C Terminus)
Human Gene ID(s)	255189
Non-Human GeneID(s)
Important Comments
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA
Tested Species
Peptide ELISA	antibody detection limit dilution 1 to 16000.
Western Blot	Preliminary experiments gave bands at approx 55-60kDa and 35kDa in Human Placenta lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculat
Reference(s)	Ohto T, Uozumi N, Hirabayashi T, Shimizu T, Identification of novel cytosolic phospholipase A(2)s, murine cPLA(2){delta}, {epsilon}, and {zeta}, which form a gene cluster with cPLA(2){beta}. The Journal of biological chemistry 2005 Jul 280 (26)