Goat anti-Npc1(mouse) Antibody

Item#:

dAP-2150

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-Npc1(mouse) Antibody
Principle Name	Npc1(mouse)
Official Symbol	Npc1
All Name and Symbols	Npc1; Niemann Pick type C1; A430089E03Rik; C85354; D18Ertd139e; D18Ertd723e; lcsd; nmf164; spm; niemann-Pick C1 protein; sphingomyelinosis
Accession Number(s)	NP_032746.2
Peptide Sequence	TYHTILKTSADYTD, is from internal region
Human Gene ID(s)
Non-Human GeneID(s)	18145 (mouse);
Important Comments
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA
Tested Species
Peptide ELISA	antibody detection limit dilution 1 to 16000.
Western Blot	Preliminary experiments gave an approx 36kDa band in lysates of cell lines NIH3T3 and NSO after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculate
Reference(s)	Tang Y, Leao IC, Coleman EM, Broughton RS, Hildreth JE, Deficiency of niemann-pick type C-1 protein impairs release of human immunodeficiency virus type 1 and results in Gag accumulation in late endosomal/lysosomal compartments. Journal of virology 2009 Aug 83 (16)