Goat anti-SOX3 Antibody |
| Principle Name | SOX3 |
| Official Symbol | SOX3 |
| All Name and Symbols | SOX3; SRY (sex determining region Y)-box 3; GHDX; MRGH; PHP; PHPX; SOXB; transcription factor SOX-3 |
| Accession Number(s) | NP_005625.2 |
| Peptide Sequence | DAASPLPGGRLHGVH, is from C Terminus |
| Human Gene ID(s) | 6658 |
| Non-Human GeneID(s) | |
| Important Comments | |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB, IHC |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 8000. |
| Western Blot | Approx 42kDa band observed in Human Brain (Cerebellum, Cerebral Cortex and Frontal Cortex) lysates (calculated MW of 45.2kDa according to NP_005625.2). Recommended concentration |
| Immunohistochemistry | In paraffin embedded Human Cerebrl Cortex shows nuclear staining at verious intensities along the neuronal cells. Recommended concentration, 3-5µg/ml. |
| Reference(s) | Mojsin M, Stevanovic M, PBX1 and MEIS1 up-regulate SOX3 gene expression by direct interaction with a consensus binding site within the basal promoter region. The Biochemical journal 2010 Jan 425 (1) |
