Goat anti-DAG1 Antibody |
| Principle Name | DAG1 |
| Official Symbol | DAG1 |
| All Name and Symbols | DAG1; dystroglycan 1 (dystrophin-associated glycoprotein 1); 156DAG; A3a; AGRNR; DAG; alpha-dystroglycan; beta-dystroglycan; dystroglycan 1 |
| Accession Number(s) | NP_004384.4 |
| Peptide Sequence | HVGKHEYFMHATDK, is from internal region |
| Human Gene ID(s) | 1605 |
| Non-Human GeneID(s) | |
| Important Comments | This antibody is expected to recognize both the precursor and the mature alpha-dystroglycan, but not the mature beta-dystroglycan. |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB |
| Tested Species | Human, Mouse, Rat |
| Peptide ELISA | antibody detection limit dilution 1 to 128000. |
| Western Blot | Approx 100kDa band observed in Human Skeletal Muscle lysates (calculated MW of 97.4kDa according to NP_004384.4). Approx 80kDa band observed in Mouse and Rat Skeletal Muscle lysates (calculated MW of 96.9kDa according to NP_034147.1). The o |
| Reference(s) | de Bernabé DB, Inamori K, Yoshida-Moriguchi T, Weydert CJ, Harper HA, Willer T, Henry MD, Campbell KP, Loss of alpha-dystroglycan laminin binding in epithelium-derived cancers is caused by silencing of LARGE. The Journal of biological chemistry 2009 Apr 284 (17) |
