Goat anti-BDNF Antibody |
| Principle Name | BDNF |
| Official Symbol | BDNF |
| All Name and Symbols | BDNF ; brain-derived neurotrophic factor; MGC34632; neurotrophin |
| Accession Number(s) | NP_001700.2; NP_001700.2; NP_733928.1; NP_733929.1; NP_733930.1; NP_733931.1 |
| Peptide Sequence | ETKCNPMGYTKE, is from internal region |
| Human Gene ID(s) | 627 |
| Non-Human GeneID(s) | |
| Important Comments | This antibody is expected to recognise all reported isoforms (NP_001700.2; NP_001700.2; NP_733928.1; NP_733929.1; NP_733930.1; NP_733931.1) |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, IHC, IF |
| Tested Species | Human, Mouse, Rat |
| Peptide ELISA | antibody detection limit dilution 1 to 4000. |
| Immunohistochemistry | In paraffin embedded Human Cortex shows cytoplasm staining of neuronal cells. Recommended concentration, 3-5µg/ml.
Frozen sections of Mouse sciatic nerve shows intensified staining after injury, consistent with the literature (Tonr |
| Reference(s) | Yoshii A, Constantine-Paton M. BDNF induces transport of PSD-95 to dendrites through PI3K-AKT signaling after NMDA receptor activation. Nat Neurosci. 2007 Jun;10(6) |
