Goat anti-GNAS Antibody |
| Principle Name | GNAS |
| Official Symbol | GNAS |
| All Name and Symbols | GNAS; GNAS complex locus; AHO; C20orf45; GNAS1; GPSA; GSA; GSP; MGC33735; NESP; PHP1A; PHP1B; POH; dJ309F20.1.1; dJ806M20.3.3; OTTHUMP00000031742; OTTHUMP00000196026; OTTHUMP00000196030; adenylate cyclase-stimulating G alpha protein; guanine nucleotide bi |
| Accession Number(s) | NP_000507.1; NP_001070956.1; NP_001070957.1 |
| Peptide Sequence | QAARSNSDGEKATK, is from internal region |
| Human Gene ID(s) | 2778 |
| Non-Human GeneID(s) | 14683 (mouse); 24896 (rat); |
| Important Comments | This antibody is expected to recognize all three reported isoforms NP_000507.1, NP_001070956.1 and NP_536350.2. However, in Mouse it is expected to recognize reporterd isoforms GNASL (NP_963910.1) and XLas (NP_034439.2) only. |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB |
| Tested Species | Human, Mouse, Rat |
| Peptide ELISA | antibody detection limit dilution 1 to 16000. |
| Western Blot | Approx 45kDa band observed in lysates of cell line Jurkat (calculated MW of 45.7kDa according to NP_000507.1). Recommended concentration |
| Reference(s) | Nishihara E, Amino N, Maekawa K, Yoshida H, Ito M, Kubota S, Fukata S, Miyauchi A, Prevalence of TSH receptor and Gsalpha mutations in 45 autonomously functioning thyroid nodules in Japan. Endocrine journal 2009 Dec 56 (6) |
