Goat anti-AIM2 Antibody |
| Principle Name | AIM2 |
| Official Symbol | AIM2 |
| All Name and Symbols | AIM2; absent in melanoma 2; PYHIN4 |
| Accession Number(s) | NP_004824.1 |
| Peptide Sequence | GEKLQLTSGVHSTIK, is from C Terminus |
| Human Gene ID(s) | 9447 |
| Non-Human GeneID(s) | |
| Important Comments | |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB, IHC |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 8000. |
| Western Blot | Approx 50+55kDa bands observed in lysates of Human WI-38 fibroblast after treatment with interferon beta (calculated MW of 39.0kDa according to NP_004824.1). The observed molecular weight corresponds to earlier findings in literature with |
| Immunohistochemistry | In paraffin embedded Human Spleen shows nucleolar staining in some splenocytes. Recommended concentration, 4-6µg/ml. |
| Reference(s) | Fernandes-Alnemri T, Yu JW, Datta P, Wu J, Alnemri ES, AIM2 activates the inflammasome and cell death in response to cytoplasmic DNA. Nature 2009 Mar 458 (7237) |
