Goat anti-DEFA1/3 precursor Antibody

Item#:

dAP-2055

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-DEFA1/3 precursor Antibody
Principle Name	DEFA1/3 precursor
Official Symbol	DEFA1/3
All Name and Symbols	DEFA1; defensin, alpha 1, myeloid-related sequence ; HGNC
Accession Number(s)	NP_004075.1; NP_005208.1
Peptide Sequence	DESLAPKHPGSRKN, is from internal region
Human Gene ID(s)	1667; 1668
Non-Human GeneID(s)
Important Comments	This antibody is expected to equally recognize DEFA1 precursor and DEFA3 precursor.
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB, IHC
Tested Species	Human
Peptide ELISA	antibody detection limit dilution 1 to 128000.
Western Blot	Approx 10kDa band observed in Human Spleen lysates (calculated MW of 10.2kDa according to NP_004075.1). Recommended concentration
Immunohistochemistry	Paraffin embedded Human Thymus. Recommended concentration
Reference(s)	Chang TL, Vargas J Jr, DelPortillo A, Klotman ME. Dual role of alpha-defensin-1 in anti-HIV-1 innate immunity. J Clin Invest. 2005 Mar;115(3)