Goat anti-FBXO4 Antibody |
| Principle Name | FBXO4 |
| Official Symbol | FBXO4 |
| All Name and Symbols | FBXO4; F-box protein 4; DKFZp547N213; FBX4; FLJ10141; F-box only protein 4; F-box protein Fbx4 |
| Accession Number(s) | NP_036308.1; NP_277019.1 |
| Peptide Sequence | EHTSAVNKMFSRH, is from internal region |
| Human Gene ID(s) | 26272 |
| Non-Human GeneID(s) | 106052 (mouse); 310363 (rat); |
| Important Comments | This antibody is expected to recognize isoform 1 and 2 (NP_036308.1; NP_277019.1). |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA |
| Tested Species | |
| Peptide ELISA | antibody detection limit dilution 1 to 8000. |
| Western Blot | Preliminary experiments gave an approx 48kDa band in Human Heart ysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 44.1kDa |
| Reference(s) | Barbash O, Zamfirova P, Lin DI, Chen X, Yang K, Nakagawa H, Lu F, Rustgi AK, Diehl JA, Mutations in Fbx4 inhibit dimerization of the SCF(Fbx4) ligase and contribute to cyclin D1 overexpression in human cancer. Cancer cell 2008 Jul 14 (1) |
