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Goat anti-EVI1 / AML1 Antibody
Item#:
dAP-2051
Size Price Quantity
50.0 ug $310.00
Goat anti-EVI1 / AML1 Antibody
Principle Name EVI1 / AML1
Official Symbol MECOM
All Name and Symbols MECOM; MDS1 and EVI1 complex locus; AML1-EVI-1; EVI1; MDS1; MDS1-EVI1; MGC163392; MGC97004; PRDM3; AML1-EVI-1 fusion protein; ecotropic viral integration site 1; myelodysplasia syndrome 1; myelodysplasia syndrome-associated protein 1; oncogene EVI1; zinc
Accession Number(s) NP_001098547.3; NP_005232.2; NP_004982.2; NP_001157471.1; NP_001157472.1
Peptide Sequence DKESLHSTSH, is from internal region
Human Gene ID(s) 2122
Non-Human GeneID(s) 14013 (mouse);
Important Comments This antibody is expected to recognize all reported isoforms (NP_001098547.3; NP_005232.2; NP_004982.2; NP_001157471.1; NP_001157472.1). Reported variants represent identical protein
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, WB
Tested Species Human
Peptide ELISA antibody detection limit dilution 1 to 16000.
Western Blot Approx 170kDa band observed in lysates of cell lines A549 and HepG2 (calculated MW of 126kDa according to NP_001098547.3). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources
Reference(s) Konrad TA, Karger A, Hackl H, Schwarzinger I, Herbacek I, Wieser R, Inducible expression of EVI1 in human myeloid cells causes phenotypes consistent with its role in myelodysplastic syndromes. Journal of leukocyte biology 2009 Oct 86 (4)

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