Goat anti-Aconitase 2 (aa541-555) Antibody

Item#:

dAP-2047

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-Aconitase 2 (aa541-555) Antibody
Principle Name	Aconitase 2 (aa541-555)
Official Symbol	ACO2
All Name and Symbols	ACO2; aconitase 2, mitochondrial; ACONM; MGC20605; MGC33908; aconitate hydratase; citrate hydro-lyase
Accession Number(s)	NP_001089.1
Peptide Sequence	QDTYQHPPKDSSGQH, is from internal region
Human Gene ID(s)	50
Non-Human GeneID(s)	11429 (mouse); 79250 (rat);
Important Comments
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB, IHC
Tested Species	Human, Mouse, Rat, Pig
Peptide ELISA	antibody detection limit dilution 1 to 16000.
Western Blot	Approx 90kDa band observed in Human Heart lysates, in Human, Mouse and Rat Adipose lysates and in Mouse, Rat and Pig Skeletal Muscle lysates (calculated MW of 85.4kDa according to NP_001089.1). Recommended concentration
Immunohistochemistry	In paraffin embedded Human Breast shows mitochondrial staining in the epithelial cells of the ducts. Recommended concentration, 3-5µg/ml.
Reference(s)	Bota DA, Davies KJ. Lon protease preferentially degrades oxidized mitochondrial aconitase by an ATP-stimulated mechanism. Nat Cell Biol. 2002 Sep;4(9)